Developing an efficient manipulation technique of cells is one of key issues in achieving high sensing performance of lab on a chip device. Dielectrophoresis (DEP) has many advantages for manipulating particles and cells compared with other manipulation techniques because most of cells have distinguishing dielectric properties. However, it is difficult to manipulate particles and cells actively with conventional DEP system. For active manipulation, the present study suggests a new DEP system for manipulating yeast cells with liquid electrodes. We designed a new DEP system with ITO glass and SU-8 based liquid electrode micro-channel and poly-dimethyl-siloxane (PDMS) chamber. PDMS chamber has thin PDMS layer on the bottom by PDMS squeezing method. The effect of AC electric field strength and frequency were experimented. With the designed DEP chip, yeast cells collection efficiencies and dielectrophoretic velocities were measured for various AC electric field strengths and frequencies. Consequently, by using these results, active manipulation of cells may be possible with a new liquid electrode DEP technique.