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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 76 - 81 (6page)
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초록· 키워드
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Background: Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with the Seeplex VRE ACE detection kit (Seeplex; Seegene, Korea), a conventional multiplex PCR assay.
Methods: A chromogenic agar-based culture, in which pre-selected vancomycin-resistant enterococci (VRE) was grown and subsequently plated on blood agar with vancomycin disks, was regarded as the reference method. A total of 304 consecutive rectal swab specimens were tested for VRE by culture and by iNtRON and Seeplex PCR assays. For the PCR assays, specimens were enriched for 16-24 hr before PCR.
Results: VRE were isolated from 44 (14.5%) specimens by chromogenic agar-based culture. The clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the iNtRON assay were 100% (95% confidence interval: 89.8%-100%), 99.2% (96.9%-99.9%), 95.6% (83.6%-99.2%), and 100% (98.2%-100%), respectively, while those of the Seeplex assay were 97.7% (86.2%-99.9%), 99.6% (97.5%-99.9%), 97.7% (86.2%-99.9%), and 99.6% (97.5%-99.9%), respectively. The iNtRON assay had a detection limit of 3,159 copies/μL and 13,702 copies/μL for the vanA and vanB genes, respectively. No cross-reactivity was observed in 11 non-VRE bacterial culture isolates.
Conclusions: The overall performance of the iNtRON assay was comparable to that of a chromogenic agar-based culture method for prompt identification of VRE-colonized patients in hospitals. This assay could be an alternative or supportive method for the effective control of nosocomial VRE infection.
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참고문헌 (22)
참고문헌 신청
Babady NE / 2012 / Performance characteristics of the Cepheid Xpert vanA assay for rapid identification of patients at high risk for carriage of vancomycin-resistant Enteroco
Bae MH / 2013 / Evaluation of iNtRON VRE vanA/vanB real-time PCR for follow-up surveillance of VRE-infected or colonized patients / Diagn Microbiol Infect Dis 77 : 292 ~ 295
Bell JM / 1998 / Emergence of vancomycin-resistant enterococci in Australia: phenotypic and genotypic characteristics of isolates / J Clin Microbiol 36 : 2187 ~ 2190
Bonten MJ / 1998 / The role of “colonization pressure” in the spread of vancomycin-resistant enterococci: an important infection control variable / Arch Intern Med 158 : 11
Courvalin P / 2006 / Vancomycin resistance in gram-positive cocci / Clin Infect Dis 42 (S1) : S25 ~ 34
Bae MH / 2013 / Evaluation of iNtRON VRE vanA/vanB real-time PCR for follow-up surveillance of VRE-infected or colonized patients / Diagn Microbiol Infect Dis 77 : 292 ~ 295
Bell JM / 1998 / Emergence of vancomycin-resistant enterococci in Australia: phenotypic and genotypic characteristics of isolates / J Clin Microbiol 36 : 2187 ~ 2190
Bonten MJ / 1998 / The role of “colonization pressure” in the spread of vancomycin-resistant enterococci: an important infection control variable / Arch Intern Med 158 : 11
Courvalin P / 2006 / Vancomycin resistance in gram-positive cocci / Clin Infect Dis 42 (S1) : S25 ~ 34
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