지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Objectives : The purpose of this study is to analyze the sterilizing effects of toothbrushes by administering bacteria into toothbrushes and reproducing the antibacterial effects using a microwave oven.
Methods : The heads of four-row mid-strength toothbrushes were cut, put in a bacterial solution (3×109cells/ml) for vortexing, and sterilized with microwaves for 0, 30, and 60 seconds. They were then moved into four tubes containing DW 10 ml and suspended in a vortex mixer for two minutes to separate bacteria from them. DW 9ml was added by 1ml of bacteria for dilution of 10~106 times. It was then administered to the BHI agar plate by 0.1ml and cultured at 37℃ for 24 hours. Total number of bacteria adhered to a toothbrush was obtained by multiplying the number of colonies by the dilution factor. The experiment was done in the first, second, and third step, being repeated in a normal temperature drier (23℃) after 5, 9 and 24 hours.
Results : The results of the experiment revealed that the sterilizing effects were 95% or over. When toothbrushes were sterilized for 60 seconds, the number of colonies is about 11 after drying for 5 hours, 7after drying for 9 hours and 2 after drying for 24 hours.
The sterilizing effects reached 98% when the bacteria-administered toothbrush was sterilized for 1 minute after drying for 24 hours.
Conclusions : The results demonstrated that toothbrush sterilizing by using microwave is a suitable way to prevent cross-contamination of toothbrushes by oral bacterial infection and thus easy to use at home.
However, this study suggests that toothbrush sterilizing by using microwave should be limited within two times a week because the physical properties of toothbrush might be changed.
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참고문헌 (19)
참고문헌 신청
Banik S / 2003 / Bioeffects of microwave-a brief review / Bioresource Technology 87 (2) : 155 ~ 159
Cardoso VH / 2007 / Microwave disinfection of gauze contaminated with bacteria and fungi / Indian J Med Microbiol 25 (4) : 428 ~ 429
Caudry SD / 1995 / Contaminated toothbrushes and their disinfection / J Can Dent Assoc 61 (6) : 511 ~ 516
Dreyfuss MS / 1980 / Comparison of effects of sublethal microwave radiation and conventional heating on the metabolic activity of Staphylococcus aureus / Appl Environ Microb
Guan D / 2003 / Microbiological validation of microwave-circulated water combination heating technology by inoculated pack studies / J. Food Sci 68 (4) : 1428 ~ 1432
Cardoso VH / 2007 / Microwave disinfection of gauze contaminated with bacteria and fungi / Indian J Med Microbiol 25 (4) : 428 ~ 429
Caudry SD / 1995 / Contaminated toothbrushes and their disinfection / J Can Dent Assoc 61 (6) : 511 ~ 516
Dreyfuss MS / 1980 / Comparison of effects of sublethal microwave radiation and conventional heating on the metabolic activity of Staphylococcus aureus / Appl Environ Microb
Guan D / 2003 / Microbiological validation of microwave-circulated water combination heating technology by inoculated pack studies / J. Food Sci 68 (4) : 1428 ~ 1432
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