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자료유형
학술저널
저자정보
배세지 (원광대학교) 김정희 (원광대학교)
저널정보
대한미용학회 대한미용학회지 대한미용학회지 제17권 제1호
발행연도
2021.1
수록면
17 - 23 (7page)

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LED and UV nail lamps emit UV wavelengths and work in the same way. Exposure to UV radiation can cause damage to the skin and tissues surrounding the nail. However, no comparative in vitro studies have been performed to-date determine the cytotoxic dose of UVA and UVB for keratinocytes, fibroblasts, and nail matrix cells. In the current study, we investigated the effects of UVA and UVB irradiation on human nail matrix cell, HS68, and HaCaT. To determine the cytotoxic dose of UV light (UVL), we irradiated cultured human nail matrix cell (WKUH-2020- 10-004), HS68, and HaCaT with ultraviolet light in the range of 0?20 J/cm² for UVA and 0?400 mJ/cm² for UVB. The required UVL was supplied by the Waldmann UV109 model which delivered uniform irradiation at a distance of 2 cm. The viability of UV-treated cells was assessed via MTT-assay. Results indicated that the cell viability rate of UVA-treated nail matrix cells was 90% for various doses. The viability of the nail matrix cells irradiated with 400 mJ/cm² UVB was 63% compared to the non-irradiated cells. The viability rate of HS68 cells after irradiation with UVA and UVB for all doses was more than 80% compared to the control. For a dose of 20 J/cm² UVA, we observed a 54% cell viability. When irradiated with 100 mJ/cm² UVB, the HaCaT cells exhibited 29% cell viability. Finally, in conclusion it can be said that UVB has a more harmful effect on cell viability than UVA. In addition, UVB has the most harmful effects on the HaCaT cells compared to the nail matrix and HS68 cells. Based on this, further research is needed on the effect of nail lamp wavelengths on the nail apparatus.
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