Extraction and Characterization of Human Adipose Tissue-Derived Collagen: Toward Xeno-Free Tissue Engineering

Kim Minseong; Yeo MyungGu; Lee KyoungHo; Park Min-Jeong; Han Gyeongyeop; Lee Chansong; Park Jihyo; Jung Bongsu

doi:10.1007/s13770-023-00612-2

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저자정보: Kim Minseong (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation) Yeo MyungGu (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation) Lee KyoungHo (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation) Park Min-Jeong (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation) Han Gyeongyeop (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation) Lee Chansong (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation) Park Jihyo (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation) Jung Bongsu (Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation)

저널정보: 한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제21권 제1호

발행연도: 2024.1

수록면: 97 - 109 (13page)

DOI: 10.1007/s13770-023-00612-2

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Background: Collagen is a key component of connective tissue and has been frequently used in the fabrication of medical devices for tissue regeneration. Human-originated collagen is particularly appealing due to its low immune response as an allograft biomaterial compared to xenografts and its ability to accelerate the regeneration process. Ethically and economically, adipose tissues available from liposuction clinics are a good resource to obtain human collagen. However, studies are still scarce on the extraction and characterization of human collagen, which originates from adipose tissue. The aim of this study is to establish a novel and simple method to extract collagen from human adipose tissue, characterize the collagen, and compare it with commercial-grade porcine collagen for tissue engineering applications. Methods: We developed a method to extract the collagen from human adipose tissue under quasi-Good Manufacturing Practice (GMP) conditions, including freezing the tissue, blood removal, and ethanol-based purification. Various techniques, including protein quantification, decellularization assessment, SDS-PAGE, FTIR, and CD spectroscopy analysis, were used for characterization. Amino acid composition was compared with commercial collagen. Biocompatibility and cell proliferation tests were performed, and in vitro tests using collagen sponge scaffolds were conducted with statistical analysis. Results: Our results showed that this human adipose-derived collagen was equivalent in quality to commercially available porcine collagen. In vitro testing demonstrated high cell attachment and the promotion of cell proliferation. Conclusion: In conclusion, we developed a simple and novel method to extract and characterize collagen and extracellular matrix from human adipose tissue, offering a potential alternative to animal-derived collagen for xeno-free tissue engineering applications.

Background: Collagen is a key component of connective tissue and has been frequently used in the fabrication of medical devices for tissue regeneration. Human-originated collagen is particularly appealing due to its low immune response as an allograft biomaterial compared to xenografts and its ability to accelerate the regeneration process. Ethically and economically, adipose tissues available from liposuction clinics are a good resource to obtain human collagen. However, studies are still scarce on the extraction and characterization of human collagen, which originates from adipose tissue. The aim of this study is to establish a novel and simple method to extract collagen from human adipose tissue, characterize the collagen, and compare it with commercial-grade porcine collagen for tissue engineering applications. Methods: We developed a method to extract the collagen from human adipose tissue under quasi-Good Manufacturing Practice (GMP) conditions, including freezing the tissue, blood removal, and ethanol-based purification. Various techniques, including protein quantification, decellularization assessment, SDS-PAGE, FTIR, and CD spectroscopy analysis, were used for characterization. Amino acid composition was compared with commercial collagen. Biocompatibility and cell proliferation tests were performed, and in vitro tests using collagen sponge scaffolds were conducted with statistical analysis. Results: Our results showed that this human adipose-derived collagen was equivalent in quality to commercially available porcine collagen. In vitro testing demonstrated high cell attachment and the promotion of cell proliferation. Conclusion: In conclusion, we developed a simple and novel method to extract and characterize collagen and extracellular matrix from human adipose tissue, offering a potential alternative to animal-derived collagen for xeno-free tissue engineering applications.

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